Equipment and protocols

Leica SP5 AOBS

Spectral scanning confocal microscope with 3 channel filterless emission pathway and transmitted light detection.

Lasers:

405 Diode
445 Diode
488/514/532 Argon ion
561 DPSS
633 HeNe

Objectives:

63x Plan Apo 1.4 na oil
63x Plan Apo 1.3 na glycerin 21°C
63x Plan Apo 1.2 na water
40x Plan Apo 0.75 na oil
20x Plan Apo 0.7 na imm

Stage:

Marzhauser motorized stage with Super-Z drive

 

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