Equipment and protocols

Microscopes and cameras

BioRad 1024 Point Scanning Confocal Microscope

Leica SP5 AOBS Point Scanning Spectral Confocal Microscope

Yokogawa CSU-10 spinning disk confocal microscope with Micropoint FRAP/Activation and DualView

Yokogawa CSU-X spinning disk confocal microscope with FLIM detection

Benchtop TIRF microscope with galvometer beam aiming

Dissecting fluorescence microscope

Cell Injection and Electrophysiology

Injection equipment and technique

Stage chambers for perfusion

Transformation

Dunk/Spray protocol for expression library transformation

Software

Metamorph - Molecular Devices

Microscope automation and image acquisition

Slidebook - Intelligent Imaging Innovations

Microscope automation and image acquisition

LAS - Leica

Microscope automation and image acquisition

Lasersharp (OS/2) - BioRad

    Acquisition of confocal images, 3D reconstructions, simple measurements.

ImageJ (Mac) - Freeware. Wayne Rasband, NIH.

    Analysis of images, reslicing of stacks, 3D reconstruction, conversion of BioRad stacks to Quicktime format, much more.

Matlab - The Math Works Inc.

    Image processing and analysis, custom programming.

Imaris - Bitplane

Image processing and analysis, 3D rendering and quantitation. Integration with Matlab.

Volocity - Improvision

Image processing and analysis, 3D rendering and quantitation. Widefield deconvolution.

 

 

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