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pEZT expression vectors - info |
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The sequences and maps presented here were assembled by piecing together bits of sequence from a variety of sources. The overall structure of the plasmids is correct, but we can’t vouch for the entire sequence. Here are some other important points about the sequences:
- There are two junction areas for which no good sequence could be found. These short (8–12 bp) stretches of sequence are represented on the sequence maps by a string of 9-10 A-T base pairs and the label ‘unknown seq’.
- The region flanked by the t-DNA borders in the pEZT vectors was cloned non-directionally into the pBBR1MCS-5 backbone (on a Sac I fragment), and I haven’t determined which orientation it is. The direction shown in the maps was chosen arbitrarily.
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The following plasmids were used in construction of these vectors:
- pEGFP-N1/pEGFP-C1 EGFP sequence and the MCS
CLONTECH
see CLONTECH Web Site for complete sequence info
- pART7 35S promoter and ocs 3’ sequence
Bart Janssen/Brett Morris
Brett Morris
Hort+Research
Private Bag 92169
Auckland, New Zealand
- pBBR1MCS-5 broad host range plasmid
Michael E. Kovach
School of Medicine in Shreveport
Louisiana State University Medical Center
1501 Kings Highway
PO Box 33932
Shreveport, LA 71130-3932
Kovach, M., et. al. (1995) Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-6.
- pDHB321.1 Basta cassette and T-DNA ends
David Bouchez
Laboratoire de Biologie Cellulaire
INRA-Centre de Versailles F-78926 Versailles Cedex FRANCE
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Notes:
Some common Agrobacterium strains used for transformation are Gm resistant (i.e. GV3101).
Use Gm at 15 µg/ml. The low copy plasmid will not provide adequate resistence to 50 µg/ml.
David Ehrhardt and Patrick Eiken ehrhardt@andrew2.stanford.edu
Carnegie Intitution of Washington
6/1/98
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